This option can be configured under Adjust CDS boundaries by up to x bp to match nearest ORF. The boundaries of CDS annotations can be automatically adjusted to fit the closest open reading frame, if this is within a specified distance of the Source CDS. *To use unannotated sequences in the Source folder (Geneious Prime 2019.2 onwards), turn on the option “ Unannotated sequences (transferred as Misc Feature type annotations) ”. Geneious will then treat sequences without any annotations as though they have an annotation of type ”misc feature” across the full length of the sequence, with the same name as the sequence name, and this will be transferred if there is a match. The Advanced option (next to Apply button) enables you to restrict the operation to particular Annotation types, set Index lengths or adjust parameters for CDS boundaries and best matches. Transferred annotations will then appear with a comprehensive selection of Annotation qualifiers, detailing the source of the transferred annotation, and a hyperlink allowing you to view an alignment of the target region and matching annotation. If you only want to apply some of the annotations, select the annotations you want (either directly on the sequence or in the annotations table) before you click Apply. Click Apply to add the annotations to your sequences (the annotations should then appear in a darker color). These will appear faded out when they are previewed.Ħ. If necessary, adjust the Similarity slider until you see a preview of the annotations on your sequence. All primer annotations covering the same region are always annotated.ĥ. If multiple annotations in the source database (of the same type) overlap with each other in the same region on the target sequence, then only the closest match of these is annotated.
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